MDM2 antagonists promote CRISPR/Cas9-mediated precise genome editing in sheep primary cells

• Main Authors: Yan Li (Author), Di Lian (Author), Jiahao Wang (Author), Yue Zhao (Author), Yao Li (Author), Guoshi Liu (Author), Sen Wu (Author), Shoulong Deng (Author), Xuguang Du (Author), Zhengxing Lian (Author)
• Format: Book
• Published: Elsevier, 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

CRISPR-Cas9-mediated genome editing in sheep is of great use in both agricultural and biomedical applications. While targeted gene knockout by CRISPR-Cas9 through non-homologous end joining (NHEJ) has worked efficiently, the knockin efficiency via homology-directed repair (HDR) remains lower, which...