In vivo Editing of the Human Mutant Rhodopsin Gene by Electroporation of Plasmid-based CRISPR/Cas9 in the Mouse Retina

• Main Authors: Maria Carmela Latella (Author), Maria Teresa Di Salvo (Author), Fabienne Cocchiarella (Author), Daniela Benati (Author), Giulia Grisendi (Author), Antonella Comitato (Author), Valeria Marigo (Author), Alessandra Recchia (Author)
• Format: Book
• Published: Elsevier, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The bacterial CRISPR/Cas system has proven to be an efficient tool for genetic manipulation in various organisms. Here we show the application of CRISPR-Cas9 technology to edit the human Rhodopsin (RHO) gene in a mouse model for autosomal dominant Retinitis Pigmentosa. We designed single or double s...