Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq
Introduction: A protocol for testing cerebrospinal fluid specimens using RT-PCR for the diagnosis of central nervous system infections was developed 2 and used to test 20 CSF specimens. Objectives: A-Evaluation of efficiency of In-house prepared Extraction reagent. BAmplified of Enteroviral-RNA geno...
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College of Pharmacy / Mustansiriyah University,
2008-06-01T00:00:00Z.
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LEADER | 00000 am a22000003u 4500 | ||
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001 | doaj_8ff2ca40b0a24ef78eaf06da49c0acd1 | ||
042 | |a dc | ||
100 | 1 | 0 | |a Faiza L. Tuama |e author |
700 | 1 | 0 | |a Yassen I. Omeran |e author |
700 | 1 | 0 | |a Manal Khalid Abdul Ridha |e author |
700 | 1 | 0 | |a Walid K. Ridha |e author |
245 | 0 | 0 | |a Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq |
260 | |b College of Pharmacy / Mustansiriyah University, |c 2008-06-01T00:00:00Z. | ||
500 | |a 10.32947/ajps.v5i1.388 | ||
500 | |a 1815-0993 | ||
500 | |a 2959-183X | ||
520 | |a Introduction: A protocol for testing cerebrospinal fluid specimens using RT-PCR for the diagnosis of central nervous system infections was developed 2 and used to test 20 CSF specimens. Objectives: A-Evaluation of efficiency of In-house prepared Extraction reagent. BAmplified of Enteroviral-RNA genome by single-step RT-PCR. Methods: 20 cerebrospinal fluid specimens were included. RNA was extracted from 250μl by method Chomcynki, 1993. EV-RT-PCR was performed for all specimens studied. Results: We successfully isolated total RNA from 20 CSF specimens of AM cases. Ten of 20 specimens were positive by RT-PCR. Conclusions: Single-step extraction method was allowed isolation of RNA in 4hr. and provided both high yield and purity of un-degraded RNA preparation. EV-RTPCR detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurologic syndromes. | ||
546 | |a EN | ||
690 | |a Enteroviral-associated- meningitis, EV-RT-PCR | ||
690 | |a Pharmacy and materia medica | ||
690 | |a RS1-441 | ||
655 | 7 | |a article |2 local | |
786 | 0 | |n Al-Mustansiriyah Journal of Pharmaceutical Sciences, Vol 5, Iss 1 (2008) | |
787 | 0 | |n https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/388 | |
787 | 0 | |n https://doaj.org/toc/1815-0993 | |
787 | 0 | |n https://doaj.org/toc/2959-183X | |
856 | 4 | 1 | |u https://doaj.org/article/8ff2ca40b0a24ef78eaf06da49c0acd1 |z Connect to this object online. |