Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq

Introduction: A protocol for testing cerebrospinal fluid specimens using RT-PCR for the diagnosis of central nervous system infections was developed 2 and used to test 20 CSF specimens. Objectives: A-Evaluation of efficiency of In-house prepared Extraction reagent. BAmplified of Enteroviral-RNA geno...

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Main Authors: Faiza L. Tuama (Author), Yassen I. Omeran (Author), Manal Khalid Abdul Ridha (Author), Walid K. Ridha (Author)
Format: Book
Published: College of Pharmacy / Mustansiriyah University, 2008-06-01T00:00:00Z.
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001 doaj_8ff2ca40b0a24ef78eaf06da49c0acd1
042 |a dc 
100 1 0 |a Faiza L. Tuama  |e author 
700 1 0 |a Yassen I. Omeran  |e author 
700 1 0 |a Manal Khalid Abdul Ridha  |e author 
700 1 0 |a Walid K. Ridha  |e author 
245 0 0 |a Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq 
260 |b College of Pharmacy / Mustansiriyah University,   |c 2008-06-01T00:00:00Z. 
500 |a 10.32947/ajps.v5i1.388 
500 |a 1815-0993 
500 |a 2959-183X 
520 |a Introduction: A protocol for testing cerebrospinal fluid specimens using RT-PCR for the diagnosis of central nervous system infections was developed 2 and used to test 20 CSF specimens. Objectives: A-Evaluation of efficiency of In-house prepared Extraction reagent. BAmplified of Enteroviral-RNA genome by single-step RT-PCR. Methods: 20 cerebrospinal fluid specimens were included. RNA was extracted from 250μl by method Chomcynki, 1993. EV-RT-PCR was performed for all specimens studied. Results: We successfully isolated total RNA from 20 CSF specimens of AM cases. Ten of 20 specimens were positive by RT-PCR. Conclusions: Single-step extraction method was allowed isolation of RNA in 4hr. and provided both high yield and purity of un-degraded RNA preparation. EV-RTPCR detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurologic syndromes. 
546 |a EN 
690 |a Enteroviral-associated- meningitis, EV-RT-PCR 
690 |a Pharmacy and materia medica 
690 |a RS1-441 
655 7 |a article  |2 local 
786 0 |n Al-Mustansiriyah Journal of Pharmaceutical Sciences, Vol 5, Iss 1 (2008) 
787 0 |n https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/388 
787 0 |n https://doaj.org/toc/1815-0993 
787 0 |n https://doaj.org/toc/2959-183X 
856 4 1 |u https://doaj.org/article/8ff2ca40b0a24ef78eaf06da49c0acd1  |z Connect to this object online.