Diagnosis of Enteroviral-associated-Meningitis by RT-PCR for the First Time in Iraq

• Asıl Yazarlar: Faiza L. Tuama (Yazar), Yassen I. Omeran (Yazar), Manal Khalid Abdul Ridha (Yazar), Walid K. Ridha (Yazar)
• Materyal Türü: Kitap
• Baskı/Yayın Bilgisi: College of Pharmacy / Mustansiriyah University, 2008-06-01T00:00:00Z.
• Konular: article
• Online Erişim: Connect to this object online.
• Notes: 10.32947/ajps.v5i1.388; 1815-0993; 2959-183X
• Keywords: Enteroviral-associated- meningitis, EV-RT-PCR; Pharmacy and materia medica; RS1-441
• Source: Al-Mustansiriyah Journal of Pharmaceutical Sciences, Vol 5, Iss 1 (2008)

Introduction: A protocol for testing cerebrospinal fluid specimens using RT-PCR for the diagnosis of central nervous system infections was developed 2 and used to test 20 CSF specimens. Objectives: A-Evaluation of efficiency of In-house prepared Extraction reagent. BAmplified of Enteroviral-RNA genome by single-step RT-PCR. Methods: 20 cerebrospinal fluid specimens were included. RNA was extracted from 250μl by method Chomcynki, 1993. EV-RT-PCR was performed for all specimens studied. Results: We successfully isolated total RNA from 20 CSF specimens of AM cases. Ten of 20 specimens were positive by RT-PCR. Conclusions: Single-step extraction method was allowed isolation of RNA in 4hr. and provided both high yield and purity of un-degraded RNA preparation. EV-RTPCR detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurologic syndromes.