VSV-G-Enveloped Vesicles for Traceless Delivery of CRISPR-Cas9

• Main Authors: Claudia Montagna (Author), Gianluca Petris (Author), Antonio Casini (Author), Giulia Maule (Author), Gian Marco Franceschini (Author), Ilaria Zanella (Author), Luciano Conti (Author), Francesca Arnoldi (Author), Oscar R. Burrone (Author), Lorena Zentilin (Author), Serena Zacchigna (Author), Mauro Giacca (Author), Anna Cereseto (Author)
• Format: Book
• Published: Elsevier, 2018-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The method of delivery of CRISPR-Cas9 into target cells is a strong determinant of efficacy and specificity in genome editing. Even though high efficiency of Cas9 delivery is necessary for optimal editing, its long-term and high levels of expression correlate with increased off-target activity. We d...