SpyDisplay: A versatile phage display selection system using SpyTag/SpyCatcher technology

• Main Authors: Sarah-Jane Kellmann (Author), Christian Hentrich (Author), Mateusz Putyrski (Author), Hanh Hanuschka (Author), Manuel Cavada (Author), Achim Knappik (Author), Francisco Ylera (Author)
• Format: Book
• Published: Taylor & Francis Group, 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

ABSTRACTPhage display is an established method for the in vitro selection of recombinant antibodies and other proteins or peptides from gene libraries. Here we describe SpyDisplay, a phage display method in which the display is achieved via SpyTag/SpyCatcher protein ligation instead of genetically f...