Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR

Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR

OBJECTIVE: The aim of this study was to evaluate, by PCR-RFLP and real-time PCR, the yield and quality of genomic DNA collected from buccal cells by mouthwash after different storage times at room temperature. MATERIAL AND METHODS: A group of volunteers was recruited to collect buccal cells using a...

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Main Authors: Erika Calvano Küchler (Author), Patricia Nivoloni Tannure (Author), Priscila Falagan-Lotsch (Author), Taliria Silva Lopes (Author), Jose Mauro Granjeiro (Author), Lidia Maria Fonte Amorim (Author)
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Published: University of São Paulo, 2012-08-01T00:00:00Z.
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