Production of CMAH Knockout Preimplantation Embryos Derived From Immortalized Porcine Cells Via TALE Nucleases

Although noncancerous immortalized cell lines have been developed by introducing genes into human and murine somatic cells, such cell lines have not been available in large domesticated animals like pigs. For immortalizing porcine cells, primary porcine fetal fibroblasts were isolated and cultured u...

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Main Authors: JoonHo Moon (Author), Choongil Lee (Author), Su Jin Kim (Author), Ji-Yei Choi (Author), Byeong Chun Lee (Author), Jin-Soo Kim (Author), Goo Jang (Author)
Format: Book
Published: Elsevier, 2014-01-01T00:00:00Z.
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