Revisiting the Experimental Methods for Human Skin T-Cell Analysis

• Main Authors: Takuya Sato (Author), Youichi Ogawa (Author), Aoha Ishikawa (Author), Yuka Nagasaka (Author), Manao Kinoshita (Author), Ichiro Shiokawa (Author), Shinji Shimada (Author), Akira Momosawa (Author), Tatsuyoshi Kawamura (Author)
• Format: Book
• Published: Elsevier, 2022-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Tissue-resident memory T cells exist in both the epidermis and the dermis in human skin. To analyze these cells, the skin needs to be incubated with dispase II to separate the two layers, that is, the epidermis and the dermis. The next step varies among researchers; the subsequent enzymatic digestio...