A reverse transcriptase-PCR assay for detecting filarial infective larvae in mosquitoes.

• Main Authors: Sandra J Laney (Author), Caitlin J Buttaro (Author), Sabato Visconti (Author), Nils Pilotte (Author), Reda M R Ramzy (Author), Gary J Weil (Author), Steven A Williams (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2008-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>Existing molecular assays for filarial parasite DNA in mosquitoes cannot distinguish between infected mosquitoes that contain any stage of the parasite and infective mosquitoes that harbor third stage larvae (L3) capable of establishing new infections in humans. We now...