Adaptation of the intact proviral DNA assay to a nanowell-based digital PCR platform

• Main Authors: Carolin Tumpach (Author), Catherine R. Cochrane (Author), Youry Kim (Author), Jesslyn Ong (Author), Ajantha Rhodes (Author), Thomas A. Angelovich (Author), Melissa J. Churchill (Author), Sharon R. Lewin (Author), Sushama Telwatte (Author), Michael Roche (Author)
• Format: Book
• Published: Elsevier, 2023-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Quantification of intact proviruses is a critical measurement in HIV cure studies both in vitro and in vivo. The widely adopted 'intact proviral DNA assay' (IPDA), designed to discriminate and quantify genetically intact HIV proviruses based on detection of two HIV sequence-specific target...