Maintenance of mouse trophoblast stem cells in KSR-based medium allows conventional 3D culture

• Main Authors: Shuai SUN (Author), Shota YANO (Author), Momo O NAKANISHI (Author), Michiko HIROSE (Author), Kazuhiko NAKABAYASHI (Author), Kenichiro HATA (Author), Atsuo OGURA (Author), Satoshi TANAKA (Author)
• Format: Book
• Published: The Society for Reproduction and Development, 2021-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Mouse trophoblast stem cells (TSCs) can differentiate into trophoblast cells, which constitute the placenta. Under conventional culture conditions, in a medium supplemented with 20% fetal bovine serum (FBS), fibroblast growth factor 4 (FGF4), and heparin and in the presence of mouse embryonic fibrob...