User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9

• Main Authors: Liangliang Chen (Author), Ying Ye (Author), Hongxia Dai (Author), Heyao Zhang (Author), Xue Zhang (Author), Qiang Wu (Author), Zhexin Zhu (Author), Rapolas Spalinskas (Author), Wenyan Ren (Author), Wensheng Zhang (Author)
• Format: Book
• Published: Hindawi Limited, 2018-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a st...