Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids

• Main Authors: Julia Eichsteininger (Author), Kerstin Kirisits (Author), Claudia Smöch (Author), Christa Stadlbauer (Author), Chi Huu Nguyen (Author), Walter Jäger (Author), Ali Özmen (Author), Gerhard Ecker (Author), Georg Krupitza (Author), Liselotte Krenn (Author)
• Format: Book
• Published: MDPI AG, 2019-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

We investigated the effect of 21 flavonoids in a three-dimensional in vitro system for their ability to inhibit gap formation by MCF-7 breast cancer spheroids in monolayers of lymphendothelial cells. Different representatives of the classes of flavones, flavonols, and flavanones were tested in the circular chemorepellent-induced defects (CCID)-assay. Bay11-7082, a known inhibitor of CCID formation served as the positive control. This study provides the first comparison of the potential of flavonoids to suppress features influencing the intravasation of MCF-7 breast cancer cells aggregates through the lymph endothelial barrier. The most significant effects were seen after incubation with the flavones luteolin, chrysin, and apigenin. Additional hydroxylation or methoxylation in positions 6 or 8, as expected, resulted in decreased activity. The tested flavanones remained without or low efficacy.

Item Description

2218-0532
10.3390/scipharm87030023