Efficient Modification of the CCR5 Locus in Primary Human T Cells With megaTAL Nuclease Establishes HIV-1 Resistance

• Main Authors: Guillermo S Romano Ibarra (Author), Biswajit Paul (Author), Blythe D Sather (Author), Patrick M Younan (Author), Karen Sommer (Author), John P Kowalski (Author), Malika Hale (Author), Barry Stoddard (Author), Jordan Jarjour (Author), Alexander Astrakhan (Author), Hans-Peter Kiem (Author), David J Rawlings (Author)
• Format: Book
• Published: Elsevier, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A naturally occurring 32-base pair deletion of the HIV-1 co-receptor CCR5 has demonstrated protection against HIV infection of human CD4+ T cells. Recent genetic engineering approaches using engineered nucleases to disrupt the gene and mimic this mutation show promise for HIV therapy. We developed a...