ABTS On-Line Antioxidant, α-Amylase, α-Glucosidase, Pancreatic Lipase, Acetyl- and Butyrylcholinesterase Inhibition Activity of <i>Chaenomeles</i> Fruits Determined by Polyphenols and other Chemical Compounds
This study aimed to identify and quantify the chemical composition and polyphenolic profile of 19 cultivars of <i>Chaenomeles × superba</i>, <i>Chaenomeles japonic</i>a, and <i>Chaenomeles speciosa</i> by liquid chromatography coupled with photodiode array detecto...
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| Main Authors: | , , , , , |
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| Format: | Book |
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MDPI AG,
2020-01-01T00:00:00Z.
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| Summary: | This study aimed to identify and quantify the chemical composition and polyphenolic profile of 19 cultivars of <i>Chaenomeles × superba</i>, <i>Chaenomeles japonic</i>a, and <i>Chaenomeles speciosa</i> by liquid chromatography coupled with photodiode array detector and quadrupole time-of-flight electrospray ionization mass spectrometry (LC-PDA-QTOF-ESI-MS). Antioxidant (ABTS on-line, ABTS, FRAP, and ORAC), as well as in vitro biological activities, i.e., the ability to inhibit α-amylase, α-glucosidase, pancreatic lipase, acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), and 15-lipoxygenase (15-LOX) were determined. Most of the <i>Chaenomeles</i> species and cultivars analyzed in this study have not been examined in this respect until now. Fruits contained 30.26 to 195.05 mg of vitamin C, 0.65 to 1.69 g of pectin, 0.32 to 0.64 g of ash, 0.60 to 3.98 g of sugars, and 41.64 to 110.31 g of organic acids in 100 g fresh weight. The lowest content of total polyphenols showed <i>C. speciosa</i> ‘Rubra’ (57.84 g/kg dry weight, dw) while <i>C.</i> × <i>superba</i> ’Nicoline’ (170.38 g/kg dw) exhibited the highest concentration of those compounds. In the phenolic compounds, polymeric procyanidin fraction predominated (65%) with procyanidin B2, C1, and (−)-epicatechin the most abundant. The antioxidant capacity measured by ABTS assay was mainly formed by polymeric procyanidins and flavan-3-ols, which was confirmed by ABTS on-line profiling. <i>Chaenomeles</i> fruits showed high potential for inhibition of α-glucosidase and pancreatic lipase. The analyzed cultivars displayed greater potential for acetylcholinesterase (AChE) inhibition than for butyrylcholinesterase (BuChE). The data indicate that <i>Chaenomeles</i> fruits could be regarded as a promising source of bioactive functional food. |
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| Item Description: | 2076-3921 10.3390/antiox9010060 |