Gene Silencing Using 4'-thioDNA as an Artificial Template to Synthesize Short Hairpin RNA Without Inducing a Detectable Innate Immune Response

• Main Authors: Noriko Tarashima (Author), Hidenori Ando (Author), Takamitsu Kojima (Author), Nozomi Kinjo (Author), Yosuke Hashimoto (Author), Kazuhiro Furukawa (Author), Tatsuhiro Ishida (Author), Noriaki Minakawa (Author)
• Format: Book
• Published: Elsevier, 2016-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The development of a versatile technique to induce RNA interference (RNAi) without immune stimulation in vivo is of interest as existing approaches to trigger RNAi, such as small interfering RNA (siRNA) and plasmid DNA (pDNA) expressing short hairpin RNA (shRNA), present drawbacks arising from innat...