Tissue Clearing and Light Sheet Microscopy: Imaging the Unsectioned Adult Zebra Finch Brain at Cellular Resolution

• Main Authors: Mariana Diales Rocha (Author), Daniel Normen Düring (Author), Philipp Bethge (Author), Fabian F. Voigt (Author), Staffan Hildebrand (Author), Fritjof Helmchen (Author), Alexander Pfeifer (Author), Richard Hans Robert Hahnloser (Author), Manfred Gahr (Author)
• Format: Book
• Published: Frontiers Media S.A., 2019-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The inherent complexity of brain tissue, with brain cells intertwining locally and projecting to distant regions, has made three-dimensional visualization of intact brains a highly desirable but challenging task in neuroscience. The natural opaqueness of tissue has traditionally limited researchers...