Comparison of the enzymatic efficiency of Liberase TM and tumor dissociation enzyme: effect on the viability of cells digested from fresh and cryopreserved human ovarian cortex

• Main Authors: Viola Maria Schmidt (Author), Vladimir Isachenko (Author), Gunter Rappl (Author), Gohar Rahimi (Author), Bettina Hanstein (Author), Bernd Morgenstern (Author), Peter Mallmann (Author), Evgenia Isachenko (Author)
• Format: Book
• Published: BMC, 2018-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background The aim of this study was to examine the effectiveness of Tumor Dissociation Enzyme (TDE) on the viability of follicles after digestion of fresh and cryopreserved ovarian cortex fragments (OCFs). Methods Fresh and thawed OCF from 14 patients (29 ± 6 years), sized 20 to 210 mm3 we...