Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene

• Main Authors: Zrinka Didara (Author), Florian Reithofer (Author), Karina Zöttl (Author), Alexander Jürets (Author), Izabella Kiss (Author), Angela Witte (Author), Reinhard Klein (Author)
• Format: Book
• Published: Elsevier, 2023-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

DNA-targeting CRISPR-Cas systems are able to cleave dsDNA in mammalian cells. Accordingly, they have been employed to target the genomes of dsDNA viruses, mostly when present in cells in a non-replicative state with low copy numbers. However, the sheer amount of viral DNA produced within a very shor...