Site-specific Genome Editing in PBMCs With PLGA Nanoparticle-delivered PNAs Confers HIV-1 Resistance in Humanized Mice

• Main Authors: Erica B Schleifman (Author), Nicole Ali McNeer (Author), Andrew Jackson (Author), Jennifer Yamtich (Author), Michael A Brehm (Author), Leonard D Shultz (Author), Dale L Greiner (Author), Priti Kumar (Author), W Mark Saltzman (Author), Peter M Glazer (Author)
• Format: Book
• Published: Elsevier, 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Biodegradable poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) encapsulating triplex-forming peptide nucleic acids (PNAs) and donor DNAs for recombination-mediated editing of the CCR5 gene were synthesized for delivery into human peripheral blood mononuclear cells (PBMCs). NPs containing th...