CRISPR-Cas13a Cleavage of Dengue Virus NS3 Gene Efficiently Inhibits Viral Replication

• Main Authors: Hao Li (Author), Shan Wang (Author), Xue Dong (Author), Qiao Li (Author), Min Li (Author), Junfeng Li (Author), Yan Guo (Author), Xia Jin (Author), Yusen Zhou (Author), Hongbin Song (Author), Zhihua Kou (Author)
• Format: Book
• Published: Elsevier, 2020-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The CRISPR-Cas9 system has been applied to DNA editing with precision in eukaryotic and prokaryotic systems, but it is unable to edit RNA directly. A recently developed CRISPR-Cas13a system has been shown to be capable of effectively knocking down RNA expression in mammalian and plant cells. In this...