OPTIMIZATION OF SODIUM DODECYL SULPHATE AS A FORMAZAN SOLVENT AND COMPARISON OF 3-(4,-5- DIMETHYLTHIAZO-2-YL)-2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) ASSAY WITH WST-1 ASSAY IN MCF-7 CELLS

• Main Authors: Ratih Asmana Ningrum (Author), Yulaika Romadhani (Author), Popi Hadi Wisnuwardhani (Author), Adi Santoso (Author)
• Formato: Libro
• Publicado: Universitas Gadjah Mada, 2014-10-01T00:00:00Z.
• Subjects: article
• Acceso en liña: Connect to this object online.

A 3-(4,-5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is a method that used to measure cell viability. It is based on the conversion of MTT by succinic dehydrogenase enzyme into insoluble formazan. Dissolution of formazan by using proper solvent is the most important step of MTT...