Optimizing Synthetic miRNA Minigene Architecture for Efficient miRNA Hairpin Concatenation and Multi-target Gene Knockdown

• Main Authors: Francis Rousset (Author), Patrick Salmon (Author), Simon Bredl (Author), Ophélie Cherpin (Author), Marta Coelho (Author), Renier Myburgh (Author), Marco Alessandrini (Author), Michael Perny (Author), Marta Roccio (Author), Roberto F. Speck (Author), Pascal Senn (Author), Karl Heinz Krause (Author)
• Format: Book
• Published: Elsevier, 2019-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Synthetic microRNA (miRNA) minigenes (SMIGs) have a major potential for molecular therapy; however, their optimal architecture still needs to be determined. We have previously optimized the stem structure of miRNA hairpins for efficient gene knockdown. Here, we investigate the overall architecture o...