Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis.

• Main Authors: Fernanda Saloum de Neves Manta (Author), Thiago Jacomasso (Author), Rita de Cássia Pontello Rampazzo (Author), Suelen Justo Maria Moreira (Author), Najua M Zahra (Author), Stewart T Cole (Author), Charlotte Avanzi (Author), Thyago Leal-Calvo (Author), Sidra Ezidio Gonçalves Vasconcellos (Author), Phillip Suffys (Author), Marcelo Ribeiro-Alves (Author), Marco Aurelio Krieger (Author), Alexandre Dias Tavares Costa (Author), Milton Ozório Moraes (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2022-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Leprosy is a chronic dermato-neurological disease caused by Mycobacterium leprae, an obligate intracellular bacterium. Timely detection is a challenge in leprosy diagnosis, relying on clinical examination and trained health professionals. Furthermore, adequate care and transmission control depend on...