A carbon nanoparticle-peptide fluorescent sensor custom-made for simple and sensitive detection of trypsin

• Main Authors: Shanshan Hou (Author), Tingting Feng (Author), Na Zhao (Author), Jiaxin Zhang (Author), Huibin Wang (Author), Ning Liang (Author), Longshan Zhao (Author)
• Format: Book
• Published: Elsevier, 2020-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Herein, we report a novel sensor to detect trypsin using a purpose-designed fluorescein-labelled peptide with negatively charged carbon nanoparticles (CNPs) modified by acid oxidation. The fluorescence of the fluorescein-labelled peptide was quenched by CNPs. The sensor reacted with trypsin to cleav...