Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

• Main Authors: Sriram Vaidyanathan (Author), Krist T. Azizian (Author), A.K.M. Ashiqul Haque (Author), Jordana M. Henderson (Author), Ayal Hendel (Author), Sabrina Shore (Author), Justin S. Antony (Author), Richard I. Hogrefe (Author), Michael S.D. Kormann (Author), Matthew H. Porteus (Author), Anton P. McCaffrey (Author)
• Format: Book
• Published: Elsevier, 2018-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The Cas9/guide RNA (Cas9/gRNA) system is commonly used for genome editing. mRNA expressing Cas9 can induce innate immune responses, reducing Cas9 expression. First-generation Cas9 mRNAs were modified with pseudouridine and 5-methylcytosine to reduce innate immune responses. We combined four approach...