Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

• Үндсэн зохиолчид: Sriram Vaidyanathan (Зохиогч), Krist T. Azizian (Зохиогч), A.K.M. Ashiqul Haque (Зохиогч), Jordana M. Henderson (Зохиогч), Ayal Hendel (Зохиогч), Sabrina Shore (Зохиогч), Justin S. Antony (Зохиогч), Richard I. Hogrefe (Зохиогч), Michael S.D. Kormann (Зохиогч), Matthew H. Porteus (Зохиогч), Anton P. McCaffrey (Зохиогч)
• Формат: Ном
• Хэвлэсэн: Elsevier, 2018-09-01T00:00:00Z.
• Нөхцлүүд: article
• Онлайн хандалт: Connect to this object online.

The Cas9/guide RNA (Cas9/gRNA) system is commonly used for genome editing. mRNA expressing Cas9 can induce innate immune responses, reducing Cas9 expression. First-generation Cas9 mRNAs were modified with pseudouridine and 5-methylcytosine to reduce innate immune responses. We combined four approach...