Inter-assay variability of next-generation sequencing-based gene panels

• Main Authors: Pham Nguyen Quy (Author), Keita Fukuyama (Author), Masashi Kanai (Author), Tadayuki Kou (Author), Tomohiro Kondo (Author), Masahiro Yoshioka (Author), Junichi Matsubara (Author), Tomohiro Sakuma (Author), Sachiko Minamiguchi (Author), Shigemi Matsumoto (Author), Manabu Muto (Author)
• Format: Book
• Published: BMC, 2022-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Tumor heterogeneity has been known to cause inter-assay discordance among next-generation sequencing (NGS) results. However, whether preclinical factors such as sample type, sample quality and analytical features of gene panel can affect the concordance between two different assa...