Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping.

• Main Authors: Fernanda Cristina Costa (Author), Amanda Fortes Francisco (Author), Shiromani Jayawardhana (Author), Simone Guedes Calderano (Author), Michael D Lewis (Author), Francisco Olmo (Author), Tom Beneke (Author), Eva Gluenz (Author), Jack Sunter (Author), Samuel Dean (Author), John Morrison Kelly (Author), Martin Craig Taylor (Author)
• Format: Bog
• Udgivet: Public Library of Science (PLoS), 2018-04-01T00:00:00Z.
• Fag: article
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<h4>Background</h4>Infection with Trypanosoma cruzi causes Chagas disease, a major public health problem throughout Latin America. There is no vaccine and the only drugs have severe side effects. Efforts to generate new therapies are hampered by limitations in our understanding of parasi...