Antigen Unmasking Is Required to Clinically Assess Levels and Localisation Patterns of Phospholipase C Zeta in Human Sperm

• Main Authors: Junaid Kashir (Author), Bhavesh V. Mistry (Author), Lujain BuSaleh (Author), Michail Nomikos (Author), Sarah Almuqayyil (Author), Raed Abu-Dawud (Author), Nadya AlYacoub (Author), Hamdan Hamdan (Author), Saad AlHassan (Author), F. Anthony Lai (Author), Abdullah M. Assiri (Author), Serdar Coskun (Author)
• Format: Book
• Published: MDPI AG, 2023-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Mammalian oocyte activation is initiated by intracellular calcium (Ca²⁺) oscillations, driven by the testis-specific phospholipase C zeta (PLCζ). Sperm PLCζ analysis represents a diagnostic measure of sperm fertilisation capacity. The application of antigen unmasking/retrieval...