In Vivo Non-radioactive Assessment of mGlu5 Receptor-Activated Polyphosphoinositide Hydrolysis in Response to Systemic Administration of a Positive Allosteric Modulator

• Main Authors: Anna R. Zuena (Author), Luisa Iacovelli (Author), Rosamaria Orlando (Author), Luisa Di Menna (Author), Paola Casolini (Author), Giovanni Sebastiano Alemà (Author), Gabriele Di Cicco (Author), Giuseppe Battaglia (Author), Ferdinando Nicoletti (Author)
• Format: Book
• Published: Frontiers Media S.A., 2018-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

mGlu5 receptor-mediated polyphosphoinositide (PI) hydrolysis is classically measured by determining the amount of radioactivity incorporated in inositolmonophosphate (InsP) after labeling of membrane phospholipids with radioactive inositol. Although this method is historically linked to the study of...