Use of Transient Transfection for cGMP Manufacturing of eOD-GT8 60mer, a Self-Assembling Nanoparticle Germline-Targeting HIV-1 Vaccine Candidate

• Main Authors: Vaneet K. Sharma (Author), Sergey Menis (Author), Evan T. Brower (Author), Eddy Sayeed (Author), Jim Ackland (Author), Angela Lombardo (Author), Christopher A. Cottrell (Author), Jonathan L. Torres (Author), Thomas Hassell (Author), Andrew B. Ward (Author), Vadim Tsvetnitsky (Author), William R. Schief (Author)
• Format: Book
• Published: MDPI AG, 2024-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We describe the current Good Manufacturing Practice (cGMP) production and subsequent characterization of eOD-GT8 60mer, a glycosylated self-assembling nanoparticle HIV-1 vaccine candidate and germline targeting priming immunogen. Production was carried out via transient expression in the human embry...