Preparation of Proper Immunogen by Cloning and Stable Expression of cDNA coding for Human Hematopoietic Stem Cell Marker CD34 in NIH-3T3 Mouse Fibroblast Cell Line

• Main Authors: Farzaneh Shafaghat (Author), Hajar Abbasi-Kenarsari (Author), Jafar Majidi (Author), Ali Akbar Movassaghpour (Author), Dariush Shanehbandi (Author), Tohid Kazemi (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2015-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Transmembrane CD34 glycoprotein is the most important marker for identification, isolation and enumeration of hematopoietic stem cells (HSCs). We aimed in this study to clone the cDNA coding for human CD34 from KG1a cell line and stably express in mouse fibroblast cell line NIH-3T3. Such ar...