Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells
ATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+...
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| Format: | Book |
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Elsevier,
2007-01-01T00:00:00Z.
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| 001 | doaj_a678c5e2b61946cbbcddacbbe2ec096c | ||
| 042 | |a dc | ||
| 100 | 1 | 0 | |a Daiju Yamazaki |e author |
| 700 | 1 | 0 | |a Susumu Ohya |e author |
| 700 | 1 | 0 | |a Kiyofumi Asai |e author |
| 700 | 1 | 0 | |a Yuji Imaizumi |e author |
| 245 | 0 | 0 | |a Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells |
| 260 | |b Elsevier, |c 2007-01-01T00:00:00Z. | ||
| 500 | |a 1347-8613 | ||
| 500 | |a 10.1254/jphs.SC0070080 | ||
| 520 | |a ATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+-entry through store-operated channels (SOCs). Ca2+-entry induced by ATP or 1-oleoyl-2-acetyl-sn-glycerol (OAG) together with Ca2+ was significantly larger than that by Ca2+ alone, suggesting the involvement of receptor-operated channels (ROCs) in the Ca2+-entry. Results obtained using pharmacological tools suggest that the contribution of Ca2+ sources to ATP-induced [Ca2+]i rise in t-BBEC117 is estimated as approximately 2:1:2 for Ca2+-release and Ca2+-entry though SOCs and ROCs, respectively. Keywords:: brain capillary endothelial cell, store-operated channel, receptor-operated channel | ||
| 546 | |a EN | ||
| 690 | |a Therapeutics. Pharmacology | ||
| 690 | |a RM1-950 | ||
| 655 | 7 | |a article |2 local | |
| 786 | 0 | |n Journal of Pharmacological Sciences, Vol 104, Iss 1, Pp 103-107 (2007) | |
| 787 | 0 | |n http://www.sciencedirect.com/science/article/pii/S1347861319342859 | |
| 787 | 0 | |n https://doaj.org/toc/1347-8613 | |
| 856 | 4 | 1 | |u https://doaj.org/article/a678c5e2b61946cbbcddacbbe2ec096c |z Connect to this object online. |