Improved DNA extraction technique from clot for the diagnosis of Chagas disease.

• Main Authors: Holger Mayta (Author), Yomara K Romero (Author), Alejandra Pando (Author), Manuela Verastegui (Author), Freddy Tinajeros (Author), Ricardo Bozo (Author), Josephine Henderson-Frost (Author), Rony Colanzi (Author), Jorge Flores (Author), Richard Lerner (Author), Caryn Bern (Author), Robert H Gilman (Author), Chagas Working Group in Perú and Bolivia (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater sensitivity than either buffy coat or whole blood samples....