Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol

• Main Authors: Magdalena Nowaczyk (Author), Agnieszka Malcher (Author), Agnieszka Zimna (Author), Wojciech Łabędź (Author), Łukasz Kubaszewski (Author), Wojciech Barczak (Author), Błażej Rubiś (Author), Natalia Rozwadowska (Author), Maciej Kurpisz (Author)
• Format: Book
• Published: MDPI AG, 2021-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The aim of the study was to modify human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) and demonstrate the optimal cell preparation protocol for application in post-infarction hearts. We used conditioned SkMDS/PC culture medium with α-phenyl-N-tert-butyl nitrone (PBN). SkMDS/PCs were cul...