Screening for Oxidative Stress Elicited by Engineered Nanomaterials: Evaluation of Acellular DCFH Assay

• Главные авторы: Anoop K. Pal (Автор), Dhimiter Bello (Автор), Bridgette Budhlall (Автор), Eugene Rogers (Автор), Donald K. Milton (Автор)
• Формат: ‌
• Опубликовано: SAGE Publishing, 2012-07-01T00:00:00Z.
• Предметы: article
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The DCFH assay is commonly used for measuring free radicals generated by engineered nanomaterials (ENM), a well-established mechanism of ENM toxicity. Concerns exist over susceptibility of the DCFH assay to: assay conditions, adsorption of DCFH onto ENM, fluorescence quenching and light scattering....