Neuropeptide deficient mice have attenuated nociceptive, vascular, and inflammatory changes in a tibia fracture model of complex regional pain syndrome

• Main Authors: Guo Tian-Zhi (Author), Wei Tzuping (Author), Shi Xiaoyou (Author), Li Wen-Wu (Author), Hou Saiyun (Author), Wang Liping (Author), Tsujikawa Kazutake (Author), Rice Kenner C (Author), Cheng Kejun (Author), Clark David J (Author), Kingery Wade S (Author)
• Format: Book
• Published: SAGE Publishing, 2012-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p>Distal limb fracture in man can induce a complex regional pain syndrome (CRPS) with pain, warmth, edema, and cutaneous inflammation. In the present study substance P (SP, Tac1^−/−) and CGRP receptor (RAMP1^−/−) deficient mice were used to investigate the contribution of neuropeptide signaling to CRPS-like changes in a tibia fracture mouse model. Wildtype, Tac1^−/−, and RAMP1^−/− mice underwent tibia fracture and casting for 3 weeks, then the cast was removed and hindpaw mechanical allodynia, unweighting, warmth, and edema were tested over time. Hindpaw skin was collected at 3 weeks post-fracture for immunoassay and femurs were collected for micro-CT analysis.</p> <p>Results</p> <p>Wildtype mice developed hindpaw allodynia, unweighting, warmth, and edema at 3 weeks post-fracture, but in the Tac1^−/− fracture mice allodynia and unweighting were attenuated and there was no warmth and edema. RAMP1^−/− fracture mice had a similar presentation, except there was no reduction in hindpaw edema. Hindpaw skin TNFα, IL-1β, IL-6 and NGF levels were up-regulated in wildtype fracture mice at 3 weeks post-fracture, but in the Tac1^−/− and RAMP1^−/− fracture mice only IL-6 was increased. The epidermal keratinocytes were the cellular source for these inflammatory mediators. An IL-6 receptor antagonist partially reversed post-fracture pain behaviors in wildtype mice.</p> <p>Conclusions</p> <p>In conclusion, both SP and CGRP are critical neuropeptide mediators for the pain behaviors, vascular abnormalities, and up-regulated innate immune responses observed in the fracture hindlimb. We postulate that the residual pain behaviors observed in the Tac1^−/− and RAMP1^−/− fracture mice are attributable to the increased IL-6 levels observed in the hindpaw skin after fracture.</p>

Item Description

10.1186/1744-8069-8-85
1744-8069