Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from <it>Orthosiphon stamineus</it> Benth as anti-diabetic mechanism

• Main Authors: Mohamed Elsnoussi Ali (Author), Siddiqui Mohammad Jamshed (Author), Ang Lee (Author), Sadikun Amirin (Author), Chan Sue (Author), Tan Soo (Author), Asmawi Mohd (Author), Yam Mun (Author)
• Format: Book
• Published: BMC, 2012-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary

<p>Abstract</p> <p>Background</p> <p>In the present study, we tested a 50% ethanolic extract of <it>Orthosiphon stamineus</it> plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities.</p> <p>Methods</p> <p>Bioactive flavonoid sinensetin was isolated from 50% ethanolic extract of <it>Orthosiphon stamineus</it>. The structure of this pure compound was determined on the NMR data and the α-glucosidase and α-amylase inhibitory activities of isolated sinensetin and 50% ethanolic extract of <it>Orthosiphon stamineus</it> were evaluated.</p> <p>Results</p> <p><it>In vitro</it> studies of a 50% ethanolic extract of <it>O. stamineus</it> and the isolated sinensetin compound showed inhibitory activity on α-glucosidase (IC₅₀: 4.63 and 0.66 mg/ml, respectively) and α-amylase (IC₅₀: 36.70 mg/ml and 1.13 mg/ml, respectively). Inhibition of these enzymes provides a strong biochemical basis for the management of type 2 diabetes via the control of glucose absorption.</p> <p>Conclusion</p> <p>Alpha-glucosidase and α-amylase inhibition could the mechanisms through which the 50% ethanolic extract of <it>O. stamineus</it> and sinensetin exert their antidiabetic activity, indicating that it could have potential use in the management of non-insulin-dependent diabetes.</p>

Item Description

10.1186/1472-6882-12-176
1472-6882