Engineering of near-PAMless adenine base editor with enhanced editing activity and reduced off-target

• Main Authors: Xiaofang Cao (Author), Junfan Guo (Author), Shisheng Huang (Author), Wenxia Yu (Author), Guanglei Li (Author), Lisha An (Author), Xiangyang Li (Author), Wanyu Tao (Author), Qing Liu (Author), Xingxu Huang (Author), Xiaohua Jin (Author), Xu Ma (Author)
• Format: Book
• Published: Elsevier, 2022-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

About 47% of pathogenic point mutations could be corrected by ABE-induced A·T-to-G·C conversions. However, the applications of ABEs are still hindered by undesired editing efficiency, limited editing scopes, and off-targeting effects. Here, we develop a new adenine base editor, by embedding TadA-8e...