An Escherichia coli Strain for Expression of the Connexin45 Carboxyl Terminus Attached to the 4th Transmembrane Domain

• Main Authors: Jennifer eKopanic (Author), Mona eAl-Mugotir (Author), Sydney eZach (Author), Das eSrustidhar (Author), Rosslyn eGrosely (Author), Paul eSorgen (Author)
• Format: Book
• Published: Frontiers Media S.A., 2013-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

A major problem for structural characterization of membrane proteins, such as connexins, by nuclear magnetic resonance (NMR) occurs at the initial step of the process, the production of sufficient amounts of protein. This occurs because proteins must be expressed in minimal based media. Here, we des...