Lab-scale siRNA and mRNA LNP manufacturing by various microfluidic mixing techniques - an evaluation of particle properties and efficiency

• Main Authors: David C. Jürgens (Author), Leonie Deßloch (Author), Diana Porras-Gonzalez (Author), Joshua Winkeljann (Author), Sebastian Zielinski (Author), Matthias Munschauer (Author), Andreas L. Hörner (Author), Gerald Burgstaller (Author), Benjamin Winkeljann (Author), Olivia M. Merkel (Author)
• Format: Book
• Published: Elsevier, 2023-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Lipid Nanoparticles (LNPs) are promising drug delivery systems for various RNAs such as small interfering (siRNA) and messenger RNA (mRNA). Microfluidic mixing is a common technique to encapsulate RNA in LNPs. However, high flow rates and lipid concentrations are used for LNP formation to control LN...