Interplay of heavy chain introns influences efficient transcript splicing and affects product quality of recombinant biotherapeutic antibodies from CHO cells

• Principais autores: Emma Kelsall (Autor), Claire Harris (Autor), Titash Sen (Autor), Diane Hatton (Autor), Sarah Dunn (Autor), Suzanne Gibson (Autor)
• Formato: Livro
• Publicado em: Taylor & Francis Group, 2023-12-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

ABSTRACTIntrons are included in genes encoding therapeutic proteins for their well-documented function of boosting expression. However, mis-splicing of introns in recombinant immunoglobulin (IgG) heavy chain (HC) transcripts can produce amino acid sequence product variants. These variants can affect...