Optical Bioimaging: From Living Tissue to a Single Molecule: Calcium Imaging in Blood Vessel In Situ Employing Two-Photon Excitation Fluorescence Microscopy

• Prif Awduron: Hisayuki Ohata (Awdur), Hideyuki Yamada (Awdur), Takeharu Niioka (Awdur), Masayuki Yamamoto (Awdur), Kazutaka Momose (Awdur)
• Fformat: Llyfr
• Cyhoeddwyd: Elsevier, 2003-01-01T00:00:00Z.
• Pynciau: article
• Mynediad Ar-lein: Connect to this object online.

ABSTRACT: Recent developments in optoelectronics permit real-time Ca2+ imaging of thin planes within cells utilizing laser scanning confocal microscopy (LSCM). However, a major complication associated with this imaging system involves increased phototoxicity with improved spatiotemporal resolution....