Affinity maturation of antibodies by combinatorial codon mutagenesis versus error-prone PCR

• Glavni autori: Jan Fredrik Simons (Autor), Yoong Wearn Lim (Autor), Kyle P. Carter (Autor), Ellen K. Wagner (Autor), Nicholas Wayham (Autor), Adam S. Adler (Autor), David S. Johnson (Autor)
• Format: Knjiga
• Izdano: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

In vitro affinity maturation of therapeutic monoclonal antibodies is commonly applied to achieve desired properties, such as improved binding kinetics and affinity. Currently there are no universally accepted protocols for generation of variegated antibody libraries or selection thereof. Here, we pe...