Quality control recommendations for RNASeq using FFPE samples based on pre-sequencing lab metrics and post-sequencing bioinformatics metrics

• मुख्य लेखकों: Yuanhang Liu (लेखक), Aditya Bhagwate (लेखक), Stacey J. Winham (लेखक), Melissa T. Stephens (लेखक), Brent W. Harker (लेखक), Samantha J. McDonough (लेखक), Melody L. Stallings-Mann (लेखक), Ethan P. Heinzen (लेखक), Robert A. Vierkant (लेखक), Tanya L. Hoskin (लेखक), Marlene H. Frost (लेखक), Jodi M. Carter (लेखक), Michael E. Pfrender (लेखक), Laurie Littlepage (लेखक), Derek C. Radisky (लेखक), Julie M. Cunningham (लेखक), Amy C. Degnim (लेखक), Chen Wang (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: BMC, 2022-09-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.

Abstract Background Formalin-fixed, paraffin-embedded (FFPE) tissues have many advantages for identification of risk biomarkers, including wide availability and potential for extended follow-up endpoints. However, RNA derived from archival FFPE samples has limited quality. Here we identified paramet...