Quality control recommendations for RNASeq using FFPE samples based on pre-sequencing lab metrics and post-sequencing bioinformatics metrics

• Main Authors: Yuanhang Liu (Author), Aditya Bhagwate (Author), Stacey J. Winham (Author), Melissa T. Stephens (Author), Brent W. Harker (Author), Samantha J. McDonough (Author), Melody L. Stallings-Mann (Author), Ethan P. Heinzen (Author), Robert A. Vierkant (Author), Tanya L. Hoskin (Author), Marlene H. Frost (Author), Jodi M. Carter (Author), Michael E. Pfrender (Author), Laurie Littlepage (Author), Derek C. Radisky (Author), Julie M. Cunningham (Author), Amy C. Degnim (Author), Chen Wang (Author)
• Format: Book
• Published: BMC, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background Formalin-fixed, paraffin-embedded (FFPE) tissues have many advantages for identification of risk biomarkers, including wide availability and potential for extended follow-up endpoints. However, RNA derived from archival FFPE samples has limited quality. Here we identified paramet...