Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs.

• Main Authors: Kristine J Kines (Author), Gabriel Rinaldi (Author), Tunika I Okatcha (Author), Maria E Morales (Author), Victoria H Mann (Author), Jose F Tort (Author), Paul J Brindley (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2010-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The schistosome egg represents an attractive developmental stage at which to target transgenes because of the high ratio of germ to somatic cells, because the transgene might be propagated and amplified by infecting snails with the miracidia hatched from treated eggs, and because eggs can be readily...