Understanding the three-dimensional world from two-dimensional immunofluorescent adjacent sections

• Main Authors: Sho Fujisawa (Author), Dmitry Yarilin (Author), Ning Fan (Author), Mesruh Turkekul (Author), Ke Xu (Author), Afsar Barlas (Author), Katia Manova-Todorova (Author)
• Format: Book
• Published: Elsevier, 2015-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Visualizing tissue structures in three-dimensions (3D) is crucial to understanding normal and pathological phenomena. However, staining and imaging of thick sections and whole mount samples can be challenging. For decades, researchers have serially sectioned large tissues and painstakingly reconstru...