Off- and on-target effects of genome editing in mouse embryos
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistingu...
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| Main Authors: | , , |
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| Format: | Book |
| Published: |
The Society for Reproduction and Development,
2018-12-01T00:00:00Z.
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| Online Access: | Connect to this object online. |
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| Summary: | Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based genome editing technology has enabled manipulation of the embryonic genome. Unbiased whole genome sequencing comparing parents to progeny has revealed that the rate of Cas9-induced mutagenesis in mouse embryos is indistinguishable from the background rate of de novo mutation. However, establishing the best practice to confirm on-target alleles of interest remains a challenge. We believe that improvement in editing strategies and screening methods for founder mice will contribute to the generation of quality-controlled animals, thereby ensuring reproducibility of results in animal studies and advancing the 3Rs (replacement, reduction, and refinement). |
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| Item Description: | 0916-8818 1348-4400 10.1262/jrd.2018-128 |