Development and evaluation of a droplet digital PCR assay for the diagnosis of paucibacillary leprosy in skin biopsy specimens.

• Main Authors: Xiujun Cheng (Author), Lele Sun (Author), Qing Zhao (Author), Zihao Mi (Author), Gongqi Yu (Author), Zhenzhen Wang (Author), Yonghu Sun (Author), Chuan Wang (Author), Chunhua Man (Author), Fanghui Fu (Author), Hong Liu (Author), Furen Zhang (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2019-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BACKGROUND:The reduced amounts of Mycobacterium leprae (M. leprae) among paucibacillary (PB) patients reflect the need to further optimize methods for leprosy diagnosis. An increasing number of reports have shown that droplet digital polymerase chain reaction (ddPCR) is a promising tool for diagnosi...